Research Project

Organoid Expansion

Predictive models of culture media stoichiometry for organoid expansion

In this project Cellesce sponsors a PhD studentship at Oxford University. Cellesce benefits from the research being undertaken. The project runs from October 2019 to September 2022.

The Cellesce bioreactor utilises flow to enhance oxygen delivery to cells and facilitates the removal of waste products. The design parameters and operating conditions of the bioreactor have been partially optimised using an empirical approach. To enable further optimisation, detailed spatial and temporal information regarding flow and the resulting oxygen metabolite concentrations throughout the bioreactor during the culture period is required.

This project aims to develop a mechanistic mathematical model that will give quantitative predictions for the fluid flow profiles and the oxygen and metabolite concentrations within the bioreactor. The mathematical model will be used to predict appropriate operating parameters for different tissues and different sub-categories of tumour within that tissue, based on knowledge from the initial culture condition studies carried out in standard well plates. The model will be used to optimise the bioreactor operating conditions for the patented CXP1 bioprocess and the CXP2 bioprocess currently under development.

The expected outcomes of this project are as follows:

  1. A predictive mathematical model to determine the flow of oxygen and metabolite concentration profiles throughout the Cellesce CXP1 and CXP2 bioreactors.
  2. Fundamental insights into the role of CXP1 and CXP2 bioreactor design and operating conditions on oxygen delivery to organoids.
  3. Optimisation of CXP1 and CXP2 bioreactor design parameters and operating conditions to ensure transport of oxygen to organoids within target range throughout the culture duration

Funded by: InFoMM